摘要
根据GenBank日本血吸虫铁蛋白基因(SjFer)的序列设计1对特异性引物,利用PCR从cDNA文库中扩增SjFer基因,与T载体连接并测序。常规方法构建植物表达载体SjFer/pBI121,采用根癌农杆菌介导法转化油菜子叶,组织培养卡那霉素抗性筛选,获得转化植株。转化植株通过PCR和Northern-blot杂交进行鉴定。结果表明SjFer可在油菜中正确表达。该研究为进一步研究转基因油菜工程疫苗奠定了基础。
To test expression of SjFer in Brassia napus,the specific primers was designed according to the SjFer sequence in GenBank(AF040385).SjFer was amplified by PCR with the specific primers from Sj adult worm cDNA library and ligated with PMD Simple-T-18vector,then sequenced.Recombinant plasmid pBI121/SjFer was constructed by routin methods.SjFer was transferred into Brassia napus by Agrobacterium mediated system.The regenerated transgenic Brassia napus was selected with kanamycin and confirmed by PCR,Northern-b...
出处
《中国兽医学报》
CAS
CSCD
北大核心
2008年第8期926-929,共4页
Chinese Journal of Veterinary Science
基金
湖南农业大学稳定人才基金资助项目(03WD07)
