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中药丹芍化纤胶囊对肝纤维化大鼠金属蛋白酶组织抑制因子-1表达的影响 被引量:1

Effect of Danshao Huaxian Capsules in Tissue Inhibitor of Metalloproteinase-1 Expression of Rats with Liver Fibrosis.
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摘要 目的探讨中药丹芍化纤胶囊对实验性肝纤维化大鼠金属蛋白酶组织抑制因子-1(tissue inhibi- tor of metalloproteinase-1,TIMP-1)表达的影响。方法雄性Wistar大鼠80只分为正常组、肝纤维化模型组、自然恢复组、低剂量治疗组、高剂量治疗组,除正常组外,其余采用CCl_4、饮酒、高脂低蛋白饮食等复合病因刺激制备肝纤维化动物模型8周,然后两治疗组分别予以低剂量(0.5g/kg)、高剂量(1.0g/kg)丹芍化纤胶囊灌胃治疗8周。实验结束后测定肝脏指数、血清透明质酸(hyaluronic acid,HA)及谷丙转氨酶(alanine aminotransferase,ALT),光镜下观察肝组织纤维化程度,检测尿羟脯氨酸(hydroxyproline,Hyp)排出量,同时用免疫组化SABC法检测肝组织中,TIMP-1的表达。结果治疗组与模型组、自然恢复组比较:肝脏指数、血清HA及ALT显著下降(P<0.05,P<0.01);肝纤维化程度显著减轻(P<0.05,P<0.01);尿Hyp排出明显增加(P<0.01);治疗组肝组织中TIMP-1的表达与模型组和自然恢复组比较则显著降低(P<0.05,P<0.01)。结论丹芍化纤胶囊能够抑制肝纤维化组织中,TIMP-1的表达,从而减弱了其对基质金属蛋白酶-1 (matrix metalloproteinase-1,MMP-1)的抑制作用,促进了细胞外基质的降解,可能是丹芍化纤胶囊抗肝纤维化作用的机制之一。 Objective To explore the effect of Danshao Huaxian Capsules(DHC)in tissue inhibitor of metalloproteinase-1(TIMP-1)expression of rats with liver fibrosis.Methods Eighty Wistar rats were divided into the normal,CCl_4-induced model,non-DHC,low dosage of DHC and large dosage of DHC group.Liver fi- brosis rat model was prepared by the stimulation of complex etiological factors,such as CCl_4-induced,taking al- cohols and high-lipid,low protein diet,etc.for 8 weeks.Then low(0.5g/kg)and large dosage (1.0g/kg)of DHC...
出处 《中国中西医结合杂志》 CAS CSCD 北大核心 2005年第S1期59-62,共4页 Chinese Journal of Integrated Traditional and Western Medicine
关键词 丹芍化纤胶囊 肝纤维化 金属蛋白酶组织抑制因子-1 胶原蛋白 Danshao Huaxian Capsule liver fibrosis tissue inhibitor of metalloproteinase-1 collagen protein
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