摘要
为了解溶基质素 1基因启动子区 5A 6A多态性与脑血栓形成之间的关系 ,利用聚合酶链反应和分子杂交技术对该多态性在中国北方人群脑血栓患者 (112 2例 )中的分布进行检测和分析 ,并与无脑血管病变的人群 (112 3例 )进行比较。结果发现 ,5A 6A多态性在两组人群中的分布符合Hardy Weinberg遗传平衡定律 ,脑血栓患者中5A5A、5A6A、6A6A三种基因型频率分别为 2 .5 0 %、2 7.6 3%和 6 9.88% ,对照组分别为 2 .2 3%、2 6 .18%和 71.5 9% ;两组人群 5A等位基因频率分别为 16 .31%和 15 .32 % ,均无统计学差异 (P >0 .0 5 )。结果提示 ,溶基质素 1基因启动子区 5A 6A多态性不是中国人群脑血栓形成发病的遗传学标记物。
Aim To evaluate the relationship between 5A/6A polymorphism in the promoter of stromelysin-1 gene and cerebral thrombosis in Chinese population. Methods The stromelysin-1 promoter 5A/6A polymorphism in 1 122 patients with cerebral thrombosis and 1 123 controls were screened by polymerase chain reaction (PCR), and then corresponding probe hybridization was performed. Results The distribution of 5A/6A polymorphism was in agreement with Hardy-Weinberg Equilibrium in both patients and controls. The prevalence of 5A5A, 5A6A and 6A6A genotypes were 2.50%, 27.63% and 69.88% in patients, and 2.23%, 26.18% 71.59% in controls, respectively. No significant difference was observed between these two groups (P>0.05). The 5A allele frequencies were 16.31% in patients, and 15.32% in controls. There is no dramatic distinction between cases and controls, either (P>0.05). Conclusion 5A/6A polymorphism in the promoter of stromelysin-1 gene is not a genetic marker of cerebral thrombosis in Chinese population.
出处
《中国动脉硬化杂志》
CAS
CSCD
2004年第6期716-718,共3页
Chinese Journal of Arteriosclerosis
