摘要
目的观察芪龙胶囊对大鼠凝血,纤溶系统的影响。方法小鼠眼眶取血测定凝血时间,大鼠取抗凝血测定凝血因子和优球蛋白降解时间(ELT),采用 FeCl_3所致单侧大脑中动脉凝闭所致局灶性脑缺血模型(MCAO),用发色底物法和酶联免疫分析法测定血浆中分子标志物的。结果芪龙胶囊0.84、0.42g/kg 组可明显延长正常小鼠凝血时间(P<0.01);芪龙胶囊0.6g/kg 组对大鼠凝血酶时间(TT)、凝血酶原时间(PT)、部分凝血活酶时间(APTT)均有明显延长作用,芪龙胶囊0.3g/kg 组对大鼠 TT、PT 有明显延长作用(P<0.05,P<0.01;芪龙胶囊0.6、0.3g/kg 组对正常大鼠 ELT 有明显缩短作用(P<0.05;P<0.01);芪龙胶囊0.6、0.3g/kg 组可显著降低模型血浆纤维蛋白原(Fbg)含量,提高模型血浆抗凝血酶Ⅲ(AT-Ⅲ)活性,还可显著提高模型血浆组织型纤溶酶原激活物(t-PA)活性(P<0.01),降低纤溶酶原抑制物(PAI)活性(P<0.01),提高 t-PA/PAI(P<0.01),降低纤溶酶原(Plg)活性(P<0.01),提高 D 二聚体和纤维蛋白降解产物(FDP)含量(P<0.01)。结论芪龙胶囊对凝血纤溶系统的作用可能是其脑保护作用和抗栓溶栓作用的基础。
Objective To observe the effect of Qilong Capsule(QLC)on serum coagulation-fibrinolysis system of mrs.Meth- otis Coagulation time was determined after the blood was taken out from the orbits in mice.Coagulation factors and eu- globulin lysis time(ELT)were determined from anti-coagulated blood of rats.Model of cerebral artery occlusion(MCAO) was made by FeCI_3 induced unilateral middle cerebral artery occlusion.Molecular marker was determined by substrate chromophore method and enzyme-linked immunoabsorbent(...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2004年第S1期248-252,共5页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
芪龙胶囊
凝血-纤溶系统
凝血时间
凝血因子
优球蛋白降解时间
分子标志物
Qilong Capsule
coagulation-fibrinolysis system
coagulation time
coagulation factor
euglobulin lysis time
molecular marker
