摘要
目的 探讨黄芪(AR)对脊髓继发性损伤的保护作用,并与甲基强地松龙(MP)进行对照。方法 Wistar大鼠60只,以改良Allen氏法制备脊髓打击伤模型,随机分为三组。测定不同药物处理后4 h、8 h、24 h脊髓组织线粒体SOD活性和MDA浓度以及血液流变学改变;光镜观察用药后1、2周黄芪对病理学改变的影响,同时进行联合行为学评分(CBS)。结果 黄芪处理后脊髓组织MDA浓度明显低于各时相点对照组,SOD活性显著升高(P<0.01),与MP治疗组无明显差异(P>0.05)。血液流变学指标也有所改善。病理检查发现黄芪治疗组髓鞘受损轻微,组织赦免范围增大。结论 黄芪治疗可以缓解脊髓损伤后的脂质过氧化反应,改善微循环,从而发挥脊髓保护作用。
Objective To investigate the protective effect of Astragalus root (AR) on the secondary spinal cord injury in Wistar rats and compare it with Methylprednisoline(MP) Methods Spinal cord injury in 60 adult rats were made at the T9 segment according to Allen's method. The rats were divided randomly into three groups:group A(single spinal cord injury,SCI) , group B(SCI with MP treatment group), group C(SCI with AR treatment group). The activity of SOD and the concentration of MDA of the injuried tissue of the spinal cord were measured at 4,8,24 h after different treatment. Hemorheology were evaluated by viscometer at the same time. Pathological influence of the drugs were observed respectively with microscope at 1 and 2 weeks, meanwhile, functional status were assessed by the combined behavioral score(CBS) . Results Comparison of the group C with group A and B showed that concentration of MDA of the injuried tissue of the spinal cord were significantly lower. The activity of SOD were significantly higher in group C than in group A, there were no significant difference between the group C and group B. Moreover, index of the hemorheology were improved,biopsy found that micro-injury of the myelin sheath and more survival tissue in the group C. Conclusion Astragalus Root can alleviate the lipid peroxidation, and improve microcirculation, thus show a promising future for treatment of spinal cord injury.
出处
《中国骨伤》
CAS
2003年第8期463-465,共4页
China Journal of Orthopaedics and Traumatology
关键词
脊髓损伤
黄芪
神经保护
Spinal cord injury
Astragalus root
Neuroprotection
