摘要
目的 通过对骨愈合过程中Ⅰ、Ⅱ、Ⅲ型前胶原mRNA、TGF-β1mRNA、BMP-2mRNA、VEGFmRNA表达的动态观察,揭示中药水蛭在此过程中对其的干预作用,了解水蛭的调节靶点,为中药基因组学的建构提供理论与实验依据。方法 通过在大鼠胫骨打孔的方法模拟骨Ⅱ期愈合的理想力学环境,在不同时间点,采用原位杂交方法检测上述各类nRNA的变化。结果 正常组VEGFmRNA在各时间点均无显著意义的阳性表达;模型组在不同时间点的不同细胞中表达强度不同;水蛭组较模型组有显著变化,水蛭对不同基因的作用不同,作用时间点不同、作用强度不同。结论 在促进骨愈合的意义上,水蛭对VEGF基因表达具有有益的调节作用。
Objective Ⅰ,Ⅱ,Ⅲ procollagen mRNA.TGF-β1 mRNA,BMP-2mRNA and VEGFmRNA were observed dynamically during bone healing in order to express the influences of Hirudo nipponica on this process, understand Hirudo nipponica regulation target, and create genetics of the Traditional Chinese Medicine. Methods Single factor interfere model was set up in SD rat. Selecting different time, the in situ hybridization method was adopted to detect the change of above-mentioned all kinds of mRNA. Results Ⅰ,Ⅱ,Ⅲ procollagen mRNA,TGF-β1 mRNA,BMP-2mRNA and VEGFmRNA have no remarkable positive expression in normal group;Model group express intensity different in different cell and different time; there is significant difference between Hirudo nipponica group and model group. Hirudo nipponica has different functions acts on different gene,in different function time order and different functional strength. Conclusion The application of Hirudo nipponica during fracture healing can lead to the different genes expression in species, localization, time, level and intensity. In conclusion, the using of Hirudo nipponica should be beneficial to bone repair.
出处
《中国骨伤》
CAS
2003年第9期513-515,共3页
China Journal of Orthopaedics and Traumatology
基金
国家中医药管理局资助项目(97A205)
关键词
骨折愈合
水蛭
基因表达
Fracture healing
Leeches
Gene expression
