摘要
目的探讨黄芪有效成分抗PC12细胞缺氧损伤的作用。方法超声醇水法提取黄芪有效成分,包括醇溶部分的黄酮、皂苷及水溶部分的黄芪多糖。用连二亚硫酸钠(Na2S2O4)诱导PC12细胞建立缺氧损伤模型,然后加入不同剂量的黄芪提取物,以四甲基偶氮唑盐(MTT)比色法及乳酸脱氢酶(LDH)漏出率测定法观察黄芪有效成分抗PC12细胞缺氧损伤作用。结果缺氧损伤可引起PC12细胞发生明显的损伤性变化,MTT测定的吸光度OD值下降,LDH漏出增加;在损伤后黄酮类可减少LDH漏出,增加吸光度OD值,使细胞存活率明显提高,以终浓度4mg/m l的作用最明显,在缺氧损伤后6h内抗损伤作用最佳,而多糖类此作用不明显。结论黄芪有效成分黄酮类有显著抗Na2S2O4诱导PC12细胞缺氧损伤作用。
Objective: To investigate the effect of valuable constituents of astragalus on the anoxic PC12 cells injured by sodium dithionite.Methods: Astragalus including alcoholsolubled cromarile,saponin and hydrosolubled polysaccharides were extracted by ultrasonic device with alcohol and ultrapure water.The PC12 cells were injured by sodium dithionite.The different dosages of valuable constituents of astragalus treated them before and after their anoxic injury.The effect of valuable constituents of astragalus on th...
出处
《中国优生与遗传杂志》
2008年第10期38-40,43,共4页
Chinese Journal of Birth Health & Heredity
