摘要
目的 :研究昆明小鼠 (KM小鼠 )单侧注射冰乙酸致睾丸损伤后环孢素A(CsA)对对侧睾丸生精功能和Fas系统表达的影响。 方法 :6 0只KM小鼠随机分为 4组 :A组为对照组 ,B组为单侧睾丸损伤组 ,C组为单侧睾丸损伤后 6h切除损伤睾丸组 ,D组为单侧睾丸损伤后 6h内开始腹腔注射CsA组。 4周后取对侧附睾尾 ,计数精子及其活率 ,对侧睾丸作石蜡切片苏木精 伊红染色和免疫组化链霉素抗生物素蛋白过氧化物酶连结 (SP)法检测Fas和FasL的表达。 结果 :D组附睾尾精子和活率计数显著高于B组 (P <0 .0 5 ) ,D组的FasL和Fas较B组显著降低 (2 4 .3± 7.0vs37.8± 5 .8和 17.8± 4 .3vs32 .4± 3.6 ,P <0 .0 5 )。 结论 :KM小鼠单侧睾丸损伤后CsA可以通过抑制Fas和FasL的表达 ,降低生精细胞凋亡 。
Objectives: To investigate the effects of Cyclosporin A (CsA) on spermatogenesis and expression of FasL and Fas in the contralateral testis after the unilateral testis was injured. Methods: 60 mice were randomly divided equally into groups A (control), B (the unilateral testis was injured by glacial acetic acid), C (excision of ipsilateral testis at 6 hours after the unilateral testis was injured by glacial acetic acid) and D (CsA within 6 hours after the unilateral testis was injured by glacial acetic acid). Sperm density and sperm motility were evaluated after 4 weeks. Expression of FasL and Fas was performed by immunohistochemistry (SP method). The positive cells with SP staining in seminiferous tubules were calculated. Results: Sperm density and sperm motility in group D were significantly increased compared with group B( P <0.05).Expression of FasL and Fas in group D decreased significantly compared with group B (24.3±7.0 vs 37.8±5.8 and 17.8±4.3 vs 32.4±3.6, P <0.05). Conclusions: CsA decreased expression of Fas and FasL and maintained spermatogenesis in the contralateral testis after the unilateral testis was injured by glacial acetic acid.
出处
《中华男科学杂志》
CAS
CSCD
2002年第3期201-203,206,共4页
National Journal of Andrology
