摘要
目的:探讨益气活血开窍复方脑脉康对缺血再灌注大鼠脑保护作用的机理。方法:以线栓法制成大鼠脑缺血再灌注模型,随机分为正常组、模型组、预防组、治疗组,后两组给予脑脉康混悬液灌胃,采用全自动氨基酸分析仪、干湿重法、原子吸收分光光度计测定脑组织中兴奋性氨基酸(EAA)含量、含水量及钙离子(Ca^(2+))含量,HE 染色观察脑组织形态学改变。结果:(1)中药组谷氨酸(Glu)含量下降(P<0.01),谷氨酸/γ-氨基丁酸(Glu/GABA)比值趋向于正常,脑组织含水量及Ca^(2+)含量下降(P<0.05)。(2)中药可减轻由于缺血再灌注所导致的脑细胞形态学的改变。结论:益气活血开窍复方脑脉康颗粒通过抑制 EAA 释放、Ca^(2+)内流而发挥脑保护作用。
Objective:To explore the therapeutic mechanism of Naomaikang(NMK)in protecting brain from ischemia/reperfusion injury. Methods:Rat model of brain ischemia/reperfusion was established by Longa s method.The experimental rats were divided into the nor- mal control group,the model group,the NMK preventive group and the NMK treatment group.NMK in suspension form was given by gastrogavage.The excitatory aminoacid(EAA)content,water content and calcium ion content in brain were determined by automatic amino acid an...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2002年第S1期57-60,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
本课题受陕西省卫生厅科研基金资助(No.99039)
关键词
中药
大鼠
缺血再灌注
兴奋性氨基酸
Chinese herbal medicine
rat
ischemia/reperfusion
excitory aminoacid
