摘要
目的用膜联蛋白Ｖ（Ａｎｎｅｘｉｎ Ｖ）法和碘化吡啶（ＰＩ）法两种流式细胞术定量检测肝细胞凋亡，作出应用评价，并用此方法探讨三七皂甙Ｒｇ１、 Ｒｂ１对急性肝损害的保护作用。方法用Ａｎｎｅｘｉｎ Ｖ法和ＰＩ法 检测脂多糖所致急性肝损害和 Ｒｇ１、 Ｒｂ１对肝损害的保护作用。用 ３Ｈ标记油酸法检测分泌型磷脂酶 Ａ２的活性。结果（ｌ）与ＰＩ染色法比， Ａｎｎｅｘｉｎ Ｖ法有更高的灵敏度和特异性，且仅用此法既能将正常细胞、凋亡细胞和坏死细胞区分开。（２）Ｒｇ１、 Ｒｂ１可明显降低脂多糖所致的大鼠急性肝损伤的凋亡率和坏死率（Ｐ＜ ０．０１），可明显降低ｓＰＬＡ２的活性（Ｐ＜ ０．０１）。结论（１）Ａｎｎｅｘｉｎ Ｖ 法是理想的定量检测凋亡细胞的方法。（２）Ｒｇ１、 Ｒｂ１可保护 ＬＰＳ所致的大鼠急性肝损害。
Objective To evaluate Annexin V technique for measuring hepatic apoptosis and to investigate the protective function of Rg1 and Rb1 on acute liver injury. Methods LPS-treated acute liver injury and the protective effect of Rg1 and Rb1 were assessed by Annexin V double staining and PI staining measurements. The activity of sPLA2 was measured by [3H]-labelled oleic acid method. Results Annexin V showed a higher sensitivity and specificity than PI staining. It was only the Annexin V assay that could discrimin...
出处
《中华肝脏病杂志》
CAS
CSCD
2001年第S1期24-26,共3页
Chinese Journal of Hepatology
