摘要
目的 研究强恒磁场对在体大鼠缺血再灌注损伤心肌细胞凋亡的影响。方法 采用Wistar大鼠心肌缺血再灌注模型 ,随机分 3组 :对照组、暴磁Ⅰ组、暴磁Ⅱ组。常规方法检测血清肌酸磷酸激酶(CPK)、心肌丙二醛 (MDA)含量 ,TUNEL法检测心肌细胞凋亡 ,免疫组化SABC法检测Bcl 2、Bax、Fas基因表达。结果 缺血再灌注后血清CPK、心肌MDA含量升高 ,对照组明显高于暴磁组 (P <0 .0 5 )。暴磁组心肌细胞凋亡率明显低于对照组。与对照组比较 ,暴磁组Bcl 2表达增强 ,而Bax、Fas蛋白表达下降。结论 强恒磁场能清除氧自由基、抑制在体大鼠缺血再灌注心肌细胞凋亡 ,保护缺血再灌注损伤心肌。
Objective To study the effect of intense constant magnetic field (ICMF) to the apoptosis of the myocardial cells during the period of the ischemia reperfusion(I/P) in rat in vivo. Methods The ischemia reperfusion models were made in rats in vivo. They were divided into three groups in random: a control group, two ICMF treating groups. Rutine method was used to detect blood creatine phosphate kinase(CPK) and myocardial malondialdehyde(MDA),TUNEL method to detect apoptosis of myocardial cells,immunohisto...
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2001年第6期19-21,71,共4页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
电磁场
心肌
心肌再灌注
细胞凋亡
Electrom magmetic field
Myocardium
Myocardial repertrsion
Apoptosis
