摘要
目的:改进肝细胞腹腔移植方法。方法:用胶原凝胶包埋鼠肝细胞并在体外培养。测定培养液中总蛋白(TP)和尿素氮(BUN)水平。将胶原培养液与鼠肝细胞混合后注入鼠腹腔,混合物在腹腔内形成凝胶并包埋肝细胞。用含酶的培养液将受体鼠腔内移植肝细胞洗出,再置于无葡萄糖(Glu)培养液中培养。测定培养液中Glu水平。对照组处理过程与实验组相同,但不用胶原。结果:胶原凝胶包埋肝细胞组的TP、BUN和Glu值均明显高于对照组(P<0.01)。胶原培养液与肝细胞混合物在注入腹腔24h后变为凝胶。结论:胶原凝胶可促进体外培养或腹腔移植肝细胞生长;胶原培养液腹腔注射和腹腔内胶原凝胶包埋细胞法,使肝细胞腹腔移植更简便、安全、实用。
Objective: To improve the method of abdominal transplantation of hepatocytes. Method: The rat hepatic cells were embedded in collagen gel and cultured in vitro. The total protein (TP) and BUN levels in the nutrient fluid were measured. The mixture of collagen nutrient solution and the hepatocytes was injected into abdominal cavity of receiver rat and then turned to gel there,so the hepatocytes were embedded in the gel. The hepatocye in the abdominal cavity were rinsed out by the nutrient solution containing...
出处
《中西医结合肝病杂志》
CAS
2001年第S1期9-10,共2页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
关键词
肝细胞
腹腔移植
胶原凝胶
Hepatic Cell Abdominal Transplantation Collagen Gel
