摘要
以体外成熟的猪卵母细胞为对象 ,研究不同卵龄、电场强度、脉冲次数和激活液 Ca+ + ,Mg+ +对猪卵母细胞电激活效果的影响。结果表明 ,1次脉冲就足以激活猪卵母细胞 ,电场强度以 1 2 5 0～ 1 5 0 0 V/cm为优 ,含 Ca+ + ,Mg+ + 的激活液激活效果显著高于非电解质液 ,卵龄以 5 4h为宜。
In this paper, some factors affecting electrical pulsing activation were analyzed, and the experimental procedure for activation was established. The results showed that one pulse was enough to activate pig oocytes. The best results could be obtained if the pulse strength was 1250～1500V/cm in electrolyte medium(0.3mol/L Mannitol+0.1mmol/L CaCl 2+0.1mmol/L MgSO4), the oocyte age was 54 h.
出处
《上海交通大学学报（农业科学版）》
2001年第4期254-257,共4页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
德国 BMBF基金资助 (CHN 99/ 3 10 )
关键词
猪
卵母细胞
电激活
pig
oocytes
electro activation
