摘要
目的：探讨 MEBO对烧伤病人血管内皮细胞的保护作用及其机理。方法：将烧伤血清加入 M199培养液配成 20％烧伤血清培养液 (BSM)，加入培养融合的人脐静脉内皮细胞中，设立 1、 3、 6、 12h 4个时间点，光镜下观察各时相点人脐静脉内皮细胞的形态学变化， MTT法测定细胞活力。结论： MEBO能明显改善烧伤血清所致的血管内皮细胞形态学变化，维持血管内皮细胞的完整性和连续性，并能明显降低烧伤血清对血管内皮细胞活力的影响。
Objective: To explore the mechanism of the protective action of MEBO on vascular endothelial cell of burn patients. Method: 20％ M199 burned serum culture medium was added to burned serum, then endothelial cells from human umbilical cord vein were added. Observation on the changes in morphology was done under optical microscope, at 1, 3, 6 and 12 hrs after cultivation. Cell activity was determined using MTT method. Conclusion: MEBO can improve the morphological changes caused by burned serum in endothelial cells, and reduce its effect on endothelial cell activity.
出处
《中国烧伤创疡杂志》
2001年第3期154-155,共2页
The Chinese Journal of Burns Wounds & Surface Ulcers
