摘要
目的 探讨应用血管内皮生长因子 (VEGF)基因治疗动物实验性冠状动脉闭塞的疗效。方法 中国实验用小型猪 19只 ,结扎左冠状动脉前降支中远段 ,心肌内多点注射自行构建的pcD2 hVEGF1 2 1 真核表达质粒。应用逆转录聚合酶链反应、VEGF免疫组化染色、Ⅷ因子相关抗原免疫组化染色等方法检测VEGF基因在心肌中的表达及其生物学作用 ,用常规冠状动脉造影观察VEGF基因治疗对闭塞冠脉侧支循环建立的作用。结果 转移VEGF基因后 ,小型猪心肌内VEGFmRNA高表达 ,VEGF免疫组化染色提示VEGF蛋白表达水平升高 ;Ⅷ因子相关抗原免疫组化染色显示心肌毛细血管增加 ;冠状动脉造影证明VEGF基因治疗能够促进闭塞冠脉侧支循环的建立。结论 心肌内注射pcD2 hVEGF1 2 1 真核表达质粒能够获得VEGFmRNA和蛋白的有效表达 ,促进心肌毛细血管增生 。
Objective To investigate the efficacy of vascular endothelial growth factor (VEGF) gene therapy in coronary occlusion of animal Methods 19 swine underwent left thoracotomy followed by the ligation of left anterior descending coronary artery Constructed pcD 2/hVEGF 121 eukaryotic expression plasmid was directly injected into the swine myocardium RT PCR, immunohistochemistry of VEGF and factor Ⅷ related antigen were used to detect VEGF gene expression and biological effect Coronary angiography was done to evaluate collateral circulation of the occluded artery Results High levels of VEGF mRNA and protein expression were detected There was a significant increase in the number of capillaries when compared with control group Coronary angiography showed better collateral circulation in VEGF group Conclusion Direct injection of pcD 2/hVEGF 121 eukaryotic expression plasmid can transfect the myocardium and express VEGF protein This gene product can increase capillary number and enhance collateral circulation of the occluded coronary
出处
《中国介入心脏病学杂志》
2001年第4期220-223,共4页
Chinese Journal of Interventional Cardiology
基金
国家 8 6 3计划生物技术资助项目 ( 86 3 10 2 BHIO3)
北京市自然科学基金资助项目 ( 7992 0 38)
关键词
内皮生长因子
心肌缺血/治疗
基因疗法
内皮
血管
Endothelial growth factor
Myocardial ischemia/therapy
Gene therapy
Endothelium, vascular
