摘要
冷酚法提取心房总 RNA,用 α- 32 P标记的大鼠心房肽互补 DNA探针与之杂交 ,检测ANP- m RNA的含量结果表明 ,2周大运动量训练 ,大鼠的 ANP- m RNA含量增加 ,4、6周大运动量训练大鼠的 ANP- m RNA含量减少 ;心室 RNA含量都减少。提示 。
Atrial total RNA is extracted by cold phenol and hybridized with preproANP-cDNA probe labeled with α-32P to detect its complementary RNA that is ANP-mRNA. The result shows that the ANP-mRNA content increases after two weeks maximal training, but decreases in four or six weeks. In ventricle, the ANP-mRNA content decreases in all training groups. It suggests that reasonable training load should keep in the range of proper intensity.
出处
《中国体育科技》
北大核心
2001年第4期15-17,共3页
China Sport Science and Technology
基金
吉林省科委资助项目! (970 3 71)
关键词
心钠素
心房
心室
基因表达
大运动量
natriuretic peptide
atria
ventricle
gene expression
maximal training
