摘要
目的:探讨动脉硬化兔血管中的血凝素样氧化低密度蛋白受体1(Lectin like oxidized low density tipoprotein recep-tor-1,LOX-1)蛋白及基因的表达及氟伐他汀、麝香保心丸对LOX-1蛋白及基因表达的影响。方法:将32只新西兰大白兔随机分为高脂饮食组、高脂饮食+氟伐他汀组、高脂饮食+麝香保心丸组及正常对照组,应用免疫组化测定兔胸主动脉中LOX-1的蛋白表达水平,用RT-PCR测定兔胸主动脉中LOX-1基因表达的水平。结果:高脂饮食组、氟伐他汀组及麝香保心丸组胸主动脉中均有LOX-1蛋白的表达,氟伐他汀及麝香保心丸干预组LOX-1蛋白及基因表达水平均明显低于高脂饮食组(P<0.05)。结论:动脉硬化兔LOX-1蛋白及基因表达水平明显升高,氟伐他汀及麝香保心丸能够明显抑制动脉硬化兔LOX-1蛋白及基因表达,从而可能在动脉粥样硬化的预防中起重要作用。
Objective:To explore the expression of LOX-1(lectin like oxidizeol low density lipoprotein receptor-1,LOX-1) in aorta of atherosclerotic rabbits and modulating the effect of fluvastatin and Shexiang baoxin pill on the gene and protein expression of LOX-1. Methods:Tirty-two New Zealand white rabbits were randomly divided into four groups: hypercholesterol diet group (fed mth high cholesterol diet),hypercholesterol diet plus Fhevastatin group(fed with high cholesterol diet plus Fhe vastain 25mgl kgld).hypercholesterol diet plus shexiang Bao xin Pill group (fed with high cholesterol diet plus shegxiang Baoxinpill 25mgl kgld).normal diet gwup(fed mith regnlar chow),the protein expression of LOX-1 in aorta of rabbits were examined by immunohistochemsthy,the gene expresswin of LOX-1 in aorte of rabbits were examined by R7-PCK. Results:hypercholesterol diet group(fed with high cholesterol diet),hypercholesterol diet plus fluvastatin group(fed with high cholesterol diet plus Fluvastatin 25mg/kg·d), hypercholesterol diet plus Shexiang baoxin pill group(fed with high cholesterol diet plus Shexiang Baoxin Pill 25mg/kg·d),normal diet group(fed with regular chow),the protein expression of LOX-1 in aorta of rabbits were examined by immunohistochemistry,the gene expression of LOX-1 in aorta of rabbits were examined by RT-PCR. Conclusion:LOX-1 was expressed in rabbit aorta of hypercholesterol diet group and two treatment groups, expression of LOX-1 in two treatment groups both significantly reduced compared with hypercholesterol diet group(P<0.05). Conclusion:The gene and protein expression levels of LOX-1 in aorta of atherosclerotic rabbits significantly increased in hypercholesterol diet group,and fluvastatin and Shexiang baoxin pill could strongly inhibit LOX-1 expression in aorta of atherosclerotic rabbits,which may play an important role in prevention of atherosclerosis.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第z1期37-40,共4页
Journal of Chongqing Medical University
基金
山东省科技厅自然科学基金项目(编号:03Bs037)
