摘要
考察了双亲灭活条件、聚乙二醇(PEG)相对分子质量、PEG浓度、融合时间、融合温度及无机离子浓度对褐黄孢链霉菌(Streptomyces gilvosporeus)SG-2002-1和SG-2002-2原生质体融合率的影响。分别采用紫外灭活和热灭活法,灭活条件为紫外灭活2 min,热灭活为60℃水浴1 h。原生质体融合最佳条件为:50%的PEG 6000,融合温度25℃,融合时间为8 min,CaCl2浓度为0.05 mol/L,MgCl2浓度为0.02 mol/L,在此条件下融合率可达5.0%。
The important factors including biparental inactivation,PEG relative molecular weight,PEG concentration,the fusion time,the fusion temperature,the concentration of inorganic ion are studied for protoplast fusion of S.gilvosporeus SG-2002-1 and S.gilvosporeus SG-2002-2.Protoplasts are completely inactivated by UV for 2 min and heat for 1 h at 60℃,respectively.The optimum conditions of protoplast fusion are as follows:PEG relative molecular weight of 6 000,PEG concentration of 50%,the fusion time of 8 min,the...
出处
《现代化工》
CAS
CSCD
北大核心
2008年第S2期349-351,353,共4页
Modern Chemical Industry
基金
天津市应用基础研究计划面上项目(07JCYBJC07800)
IFS国际科学基金(F/4239-1)
天津科技大学引进人才科研启动基金(20060426)
关键词
褐黄孢链霉菌
纳他霉素
原生质体融合
融合率
Streptomyces gilvosporeus
natamycin
protoplast fusion
fusion frequency
