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多杀性巴氏杆菌幽灵的研究 被引量：4

Preliminary study on bacterial ghost of Pasteurella multocida
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摘要 采用基因工程方法,将PhiX174噬菌体裂解基因E和温敏控制表达系统与质粒pPBA1100基因杂交,构建了重组子pPBA1100-E.将重组子转化到多杀性巴氏杆菌中,通过温度诱导使裂解基因表达.用限制性内切酶检验重组子.扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率.结果表明:重组质粒经限制性内切酶酶切鉴定,琼脂糖电泳呈现三条谱带,相对分子质量与理论值一致.扫描电镜观察显示,重组子在多杀性巴氏杆菌中成功表达,获得了细菌幽灵.菌落形成单位检验结果显示,多杀性巴氏杆菌遗传灭活率达到99%. Recombinant pPBA1100-E was constructed by which the gene E of bacteriophage Phix174 and temperature sensitivity expressing control system hybridized with plasmid pPBA1100 by genetic engineering method.Recombinant was transform to Pasteurella multocida and lysis gene E expressed by temperature inducing.Recombinant was detected by restriction endonuclease.Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi...
作者 刘东胜 朱必凤 杨旭夫
机构地区 南昌大学生命科学学院 韶关学院英东生物工程学院
出处 《韶关学院学报》 2008年第12期79-83,共5页 Journal of Shaoguan University
关键词 多杀性巴氏杆菌 细菌幽灵 裂解基因E 质粒构建 Pasteurella multocida bacterial ghost lysis gene E plasmid construction
分类号 S852.5 [农业科学—基础兽医学]
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二级参考文献26

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共引文献14

同被引文献64

  • 1顾宏伟,陆承平.兔多杀性巴氏杆菌铁调节外膜蛋白(IROMPs)抗原交叉性试验[J].微生物学报,2004,44(5):667-671. 被引量：8
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引证文献4

二级引证文献12

韶关学院学报
2008年 第12期

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