摘要
目的:探讨多种细胞因子联合刺激下,逆转录病毒载体对K562细胞的基因转移效率。方法:以PA317-GCGPXSN细胞制备病毒上清,NIH3T3细胞测定病毒滴度后,取经过SCF、IL-3、IL-6预培养刺激后的K562细胞,实施基因转移,流式细胞仪及PCR方法测定基因转移效率。结果:①病毒滴度为1.9×105CFU/m l;②对照组K562细胞测定的荧光强度数值为0.56%,实验组为77.16%,两者差异有统计学意义(P<0.01)。PCR方法扩增到了NeoR基因的特异性片断。结论:细胞因子预刺激后实施基因转移,可有效地将外源基因转移进入K562细胞基因组中。
Objective:To explore gene transfer efficiency on K562 cells mediated by retroviral vector and stimulated with cytokines.Methods: The viral supernatants were harvested with PA317-GCGPXSN and detected with NIH3T3 cells.Then gene transfer was made using K562 cells as target cells which were stimulated by SCF,IL-3 and IL-6.The gene transfer efficiency was detected by FACS and PCR.Results:The viral titer was 1.9×105 CFU/ml;The gene transfer efficiency of experiment group was 77.16%,whereas the control group was ...
出处
《西北国防医学杂志》
CAS
2008年第6期405-407,共3页
Medical Journal of National Defending Forces in Northwest China
基金
全军"十一五"杰出人才基金资助项目(06J005)
兰州军区医药科研基金资助项目(LXH-2007006)
