摘要
目的:研究过表达外源性和RNA干扰内源性NDRG3对前列腺癌细胞克隆形成的影响。方法:将NDRG3表达质粒稳定转染人类PC3前列腺癌细胞株,用1个NDRG3稳定表达亚克隆与做为对照的亲代和空质粒转染的PC3细胞进行克隆形成实验;同时利用RNA干扰技术沉默CL-1细胞的内源性NDRG3,并用Western blotting技术进行鉴定,在对照组、GFP-siRNA组和NDRG3-siRNA组进行克隆形成实验。结果:过表达外源性NDRG3的PC3细胞形成的克隆数明显多于亲代和空质粒转染的PC3细胞株;在RNA干扰实验中,CL-1对照组和GFP-siRNA组形成的总克隆数和大克隆数均明显多于NDRG3-siRNA组(P<0.05)。结论:NDRG3可以提高前列腺癌细胞的致癌潜能和克隆形成能力。
Objective To study the effect of over-expressing exogenous and RNA-interfered endogenous NDRG3 on colony-formation of prostatic carcinoma cells.Methods NDRG3 expression plasmids were stably transfected to PC3 prostatic carcinoma cell lines.The NDRG3-stable transfected PC3 sublines were studied along with parental and empty vector transfected PC3 cells as controls; Endogenous NDRG3 was silenced by RNA interference technique and appreciated using Western blotting technique.Colony-formation experiments were pe...
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2008年第6期960-963,共4页
Journal of Jilin University：Medicine Edition
基金
上海市科委自然科学基金资助课题(06ZR14072)
