摘要
目的:观察参附注射液(shenfu injection,SF)对大鼠肾缺血再灌注损伤细胞黏附分子-1(ICAM-1)、NF-κB、TNF-α表达的影响。方法:采用切除右肾,无创动脉夹夹闭左肾动脉45 min,再灌注2 h制作在体肾缺血再灌注损伤模型。36只SD大鼠随机分为缺血再灌注组、假手术对照组、参附预处理组(10 mL/kg),每组12只。免疫组化法检测肾组织中NF-κB、ICAM-1蛋白含量,酶联免疫吸附实验(ELISA)检测血清、肾组织中TNF-α的表达。结果:缺血再灌注组NF-κB、ICAM-1表达和TNF-α含量明显高于假手术对照组(P<0.01)。与缺血再灌注组相比,参附预处理组NF-κB、ICAM-1表达和TNF-α含量明显降低(P<0.01)。结论:参附通过抑制NF-κB的表达,从而下调其下游的TNF-α、ICAM-1的表达,发挥其肾脏保护作用。
AIM: To explore the effect of shenfu injection on renal ischemia reperfusion injury in rats and to observe the expression of nuclear factor kappa B(NF-κB),tumour necrosis factor-α(TNF-α) and intercellular adhension molecule-1(ICAM-1).METHODS: 36 SD rats were divided into three groups randomly.There were 12 rats in each group.Renal ischemia reperfusion injury was induced by left renal pedicle occlusion for 45 minutes,followed by reperfusion with contralateral nephrectomy in rats.In shenfu pretreatment group,...
出处
《中国临床药理学与治疗学》
CAS
CSCD
2008年第9期1005-1009,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
缺血再灌注
参附注射液
核因子-κB
细胞间黏附分子-1
肿瘤坏死因子-α
ischemia reperfusion injury
shenfu injection
nuclear factor kappa B
intercellular adhension molecule-1
tumor necrosis factor-α
