摘要
采用Folch液回流提取樱桃谷鸭肥肝脂质,脂质经氢氧化钾-三氟化硼法甲酯化后进行气相色谱分析,采用100:1分流比进样,220～240℃线性程序升温操作,BPX70(60m×0.25mmID)石英毛细管柱分离,FID检测。标准品对照结合线性程序升温等当碳长度(ECLT)值的方法定性了鸭肥肝中11种脂肪酸。面积归一法定量分析了基础日粮添加不同油脂和VE对樱桃谷鸭肥肝中脂肪酸含量变化的影响。结果表明,基础日粮中按2%水平分别添加山茶油、芝麻油以及大豆油后,肥肝不饱和脂肪酸含量均明显增加,与对照组比较均呈现极显著性差异(p<0.01),饱和脂肪酸含量明显下降,与对照组比较均呈现极显著性差异(p<0.01);基础日粮中按90IU/kg水平添加VE后,肥肝不饱和脂肪酸含量增加,与对照组比较差异显著(p<0.05),饱和脂肪酸含量下降,与对照组比较差异显著(p<0.05)。
Fatty acids in fatty liver of cherry valley duck were extracted from tissue using reflux with 'Folch' solvent,and esterified with KOH-BF3 method followed by gas chromatography analysis. A fused-silica BPX70 capillary column and FID detector were employed in gas chromatography. The inlet mode was split with split ratio of 100:1. Temperature program settings were as following:initial temperature 220 ℃,increase at 1 ℃/min to final temperature 240 ℃. 11 kinds of fatty acids in fatty liver were identified by app...
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第2期224-227,共4页
Food Science
基金
广西科学研究与技术开发计划项目(桂科攻0424001-6)
广西大学创新学分科研实践训练项目
关键词
肥肝
脂肪酸
毛细管气相色谱法
VE
饲粮油脂
fatty liver
fatty acid
capillary gas chromatography
vitamin E
dietary oil
