摘要
本实验对芝麻素脂质体进行质量评价,测定芝麻素脂质体的包封率。采用大孔树脂分离脂质体与游离药物,用高效液相色谱检测药物含量,计算包封率。该方法在5～200μg/ml范围之间线性良好(r=0.9999),RSD小于1.47%,加样回收率为98.18%。预饱和大孔树脂柱对空白脂质体的平均回收率为100.35%,且能很好的保留溶液中及脂质体制剂中的游离药物。此方法操作简单,重现性较好。
To evaluate the quality of sesamin liposome,macroporous resin was used to separate the free sesamin and HPLC was used to determine the entrapment efficiency. This method has good linearity in the range of 5 to 200 μg/ml (r=0.9999).The precision RSD is less than 1.47%,the recovery of sesamin with blank liposome is 98.18%,and the average of recovery of blank liposomes through presaturated macroporous resin is 100.35% with an excellent adsorption of free sesamin. This method is simple and reproducible.
出处
《食品科学》
CAS
CSCD
北大核心
2009年第2期232-234,共3页
Food Science
基金
天津科技大学引进人才科研启动基金项目(20060439
0200096)
