摘要
称取若干地黄样品,用去离子水定容、超声震荡、过滤后测定紫外(UV)光谱。采用多元曲线分辨-交替最小二乘法(MCR-ALS)分析测得的UV光谱数据,提取出3种纯组分的UV光谱,并计算混合体系中其相对浓度。结果表明,从混合组分中提取的UV光谱可以用来将炮制过程中混合组分简单地定性,根据这些组分相对浓度升降的变化趋势,监控地黄炮制过程,确定炮制终点。MCR-ALS-UV法为监控地黄炮制过程及确定炮制终点提供了新途径。
The UV spectra of several grams of sample of the grinded radix rehmanniae were measured after they were fixed at constant volume by deionized water,ultrasonic vibrated,and filtrated.The spectral data were then analyzed and measured by multivariate curve resolution-alternating least squares(MCR-ALS).Three UV spectral profiles of the components were directly extracted from the mixed data,and their relative concentrations were calculated.The results show that the calculated UV spectral profiles can be used to ...
出处
《计算机与应用化学》
CAS
CSCD
北大核心
2009年第3期272-274,共3页
Computers and Applied Chemistry
基金
国家自然科学基金资助项目(20675073)
河南省高校科技创新人才支持项目(2009HASTIT026).
关键词
多元曲线分辨(MCR)
交替最小二乘(ALS)
紫外光谱法
地黄
过程监控
multivariate curve resolution-alternating least square（MCR-ALS）
ultraviolet spectrometry
radix rehmanniae
process monitoring
