摘要
目的探讨大黄致结肠黑变病豚鼠血清及组织肿瘤坏死因子-α(TNF-α)水平的变化及意义。方法清洁级豚鼠112只,完全随机分为4组。正常组16只,大黄低、中、高剂量组各32只。除正常组外,大黄低、中、高剂量组分别予大黄提取液以3 g/(kg·d)、6 g/(kg·d)、12 g/(kg·d)灌胃,连续给药60天后处死各组豚鼠。应用RT-PCR法以及ELISA法,定性定量检测TNF-α水平变化及TNF-α mRNA表达。结果与正常组比较,大黄各剂量组血清、组织中TNF-α水平及TNF-α mRNA表达明显增高(P<0.01),大黄各剂量组之间比较TNF-α水平变化差异无统计学意义(P>0.05)。结论TNF-α水平随大黄致豚鼠结肠黑变病加重而呈增高趋势。
Objective To study the induced change of tumor necrosis factor α(TNF-α) level in serum and colon tissue of guinea pig model with melanosis coli and its significance induced by rhubarb(RB).MethodsOne hundred and twelve guinea pigs of clean grade were randomly divided into four groups: the 16 in the normal group(untreated) and the 3 RB groups(32 in each) treated with low(3 g/kg·d),medium(6 g/kg·d),and high(12 g/kg·d) dose of rhubarb respectively,administered by gastrogavage for 60 successive days.All guinea p...
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2009年第2期140-142,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
浙江省自然科学基金资助项目(No.X206959)
