摘要
目的:为了控制古宗金花小檗植物根的药材质量,采用反相高效液相色谱法测定药材中盐酸小檗碱的含量。方法:色谱柱Diamosil(TM)钻石C18(250mm×4.6mm,5μm)柱温25℃,乙腈-0.1%磷酸溶液(50:50)为流动相,流速1.0mL/min,检测波长265nm。结果:盐酸小檗碱在0.05075～0.45675μg范围内,线性关系良好,r=0.9999(n=5),平均加样回收率为100.86%,RSD%=1.63%。结论:本法简便,快速,准确,适用于对古宗金花小檗植物根的药材质量监控。
Objective:In order to control the Radix berberis wilsonae .var. guhtzunica raw material for medicine the quality, uses in the RP-HPLC method determination raw material for medicine the berberine hydrochloride content. Methods: Diamosil (TM) diamond C18 column (250mm×4.6mm,5μm ),column temperature was 25 ℃ , the mobile phase was acetonitrile-0.1%phosphorus acid(50:50), the speed of flow was 1.0mL/min, the UV detection wavelength was 265nm.Results: The berberine hydrochloride in 0.05075～0.45675μg scope,the li...
出处
《辽宁中医药大学学报》
CAS
2009年第3期169-170,共2页
Journal of Liaoning University of Traditional Chinese Medicine
基金
贵州中药现代化专项资助项目(2006-5019)
关键词
反相高效液相色谱法
古宗金花小檗
含量
盐酸小檗碱
RP-HPLC
Berberis wilsonae Nemsl. var. guntzunica （Ahrendt） Ahrendt
Content
Berberine hydrochloride
