摘要
制备了一种胶原-磺化羧甲基壳聚糖/硅橡胶皮肤再生材料,并以小型猪为模型,考察了其对烫伤全层皮肤缺损的修复性能.首先合成了磺化羧甲基壳聚糖,并对其结构进行了表征.制备了胶原-磺化羧甲基壳聚糖多孔支架,采用扫描电子显微镜(SEM)研究了磺化羧甲基壳聚糖含量对支架微结构的影响.随着磺化羧甲基壳聚糖含量的增大,胶原-磺化羧甲基壳聚糖支架从纤维结构向片状结构转化,且支架的孔径相对变大.采用体外成纤维细胞培养实验证明胶原-磺化羧甲基壳聚糖支架无明显细胞毒性.进一步将胶原-磺化羧甲基壳聚糖支架与硅橡胶膜复合,构建具有双层结构的皮肤再生材料.以小型猪为模型,评价了其对深度烫伤创面的修复性能.大体观察和组织学分析结果显示,胶原-磺化羧甲基壳聚糖/硅橡胶皮肤再生材料具有更快的血管化性能,且经该材料处理的创面能有效支持薄自体皮片的移植成活,实现深度烫伤创面的全层修复.
A dermal equivalent applied in the treatment of scalds has been attracting much attention.We report here the fabrication of a bilayer dermal equivalent(BDE) composed of collagen-sulfonated carboxymethyl chitosan(SCC) porous scaffold and silicone membrane,and its application for accelerating the healing of skin scalds in a Bama miniature pig model.Firstly,the SCC was synthesized,and its chemical structure was characterized by FTIR and ~1H-NMR.Then the effect of the ratio of the SCC on the microstructure of t...
出处
《高分子学报》
SCIE
CAS
CSCD
北大核心
2009年第2期111-117,共7页
Acta Polymerica Sinica
基金
国家重点基础研究发展规划(项目号2005CB623902)
浙江省科技计划(项目号2007C23014)
国家杰出青年基金(基金号50425311)资助项目
关键词
磺化羧甲基壳聚糖
胶原
皮肤再生材料
烫伤
血管化
Sulfonated carboxymethyl chitosan
Collagen
Dermal equivalent
Scald
Angiogenesis
