摘要
目的对方斑东风螺的主要过敏原进行分析、鉴定与纯化。方法通过SDS-PAGE电泳分离方斑东风螺的蛋白质组份,采用免疫印迹(Western-blotting)方法鉴定过敏原,通过离子交换层析对方斑东风螺主要过敏原进行初步纯化。结果方斑东风螺粗提液SDS-PAGE显示其主要蛋白条带主要有7条,Western-blotting显示过敏患者的阳性混合血清能与其中3个蛋白条带起反应,相对分子质量分别是56000、28000和22000。离子交换层析可初步纯化出28000和22000的过敏原蛋白。结论本实验对方斑东风螺过敏原进行了分离和鉴定,并初步纯化出方斑东风螺的主要过敏原。
Objective To isolate,identify,and purify the allergens in Babylonia areolata.Methods The total protein of the babylonia areolata was isolated by SDS-PAGE and the allergens of babylonia areolata were identified by Western blotting.To purify the allergens,ion exchange chromatography was employed.Results There were 7 main protein bands visible on the SDS-PAGE gel.The allergens whose molecular mass were 56 000,28 000,and 22 000 respectively were identified by Western blotting.The μr 28 000 and 22 000 allergens ...
出处
《免疫学杂志》
CAS
CSCD
北大核心
2009年第2期165-167,172,共4页
Immunological Journal
基金
广东省科技重点专项(2003A3080502)
深圳市科技计划(200326)
深圳大学校科研基金(200712)资助
