摘要
通过ＩＦＡ、ＶＮＴ和Ｗｅｓｔｅｒｎ－Ｂｌｏｔ等血清学试验及电镜观察，证明ＪＫ－１００株为ＰＲＲＳＶ。本病毒感染ＭＡＲＣ１４５细胞后，３６小时的ＴＣＩＤ_(50)最高，为１０^(-7.83)。把本病毒分别保存在４℃、３７℃各９天，保存在４℃９天后，ＴＣＩＤ_(50)从１０^(-7.83)下降至１０^(-7.32)，３７℃７天已测不到效价。
The JK-100 strain was identified as PRRSV by IFA,VNT,Westem-Blot and EM.The TCID50 was highest when MARC145 cell was infected by JK-100 after 36 hours.The virus was conserved under for days, it s titration reduced from 10-7.83 to 10-7.32, and under 37 for 7 days, it s titration can not be detected.
出处
《中国预防兽医学报》
CAS
CSCD
2000年第S1期37-39,共3页
Chinese Journal of Preventive Veterinary Medicine
关键词
猪生殖-呼吸综合征病毒
JK-100弱毒株
Porcine Reproductive and Respiratory Syndrome Virus
Modified JK-100 Straind
