摘要
目的 观察卵巢癌抗独特型单链抗体 ６Ｂ１１ＳｃＦｖ与鼠粒细胞巨噬细胞集落刺激因于（ｍｕｒｉｎｅ ｇｒａｎｕｌｏｃｖｔｅ－ｍａｃｒｏｐｈａｇｅ ｃｏｌｏｎｙ－ｓｔｉｍｕｌａｔｉｎｇ ｆａｃｔｏｒ．ｍＧＭ－ＣＳＦ）的融合蛋白（６Ｂ１１ｍＧＭ）在小鼠体内诱导抗肿瘤免疫反应情况，探讨此种融合蛋白作为卵巢癌疫苗的可能性及适当的免疫方法。方法 用融合蛋白６Ｂ１１ｍＧＭ免疫ＢＡＬＢ／ｃ小鼠，制备抗血清。采用间接ＥＬＩＳＡ及免疫流式方法分析抗血清特征。结果 经ＥＬＩＳＡ检测，融合蛋白６Ｂ１１ｍＧＭ免疫小鼠后，可诱导机体产生ＡＢ３。同时可刺激小鼠脾脏淋巴细胞ＣＤ４＋细胞明显升高，ＣＤ８＋细胞略有升高。结论 融合蛋白６Ｂ１１ｍＧＭ可诱导机体产生特异体液免疫和细胞免疫反应，为抗独特型卵巢癌疫苗用于临床奠定了基础。
Objective To observe whether antitumor immune response c an be induced in BALB/c mice immunized with a fusion protein(6B11mGM) of anti-idiotypic single chain antibody (6B11scFv) and murine granulocyte-macrophage colony-stimulating factor (GM-CSF) instead of ovarian carcinoma antigen.Methods BALB/c mice were immuni zed repeatedly with 6B11mGM.Indirect ELISA test and immune flow cytometer were used for analyzing the characterization of anti-idiotypic ScFv(Ab3).Result s Ab3 could be detected in the sera of immunized mice with 6B11mGM by ELISA test .Moreover,it stimulated CD4+T cell from the spleen of BALB/c mice to proliferate and CD8+ T cell to proliferate slightly.Conclusion 6B11mGM probably induce b oth humoral and cellular immunity against ovarian carcinoma in vivo .It illus tra ted a strong basic for using fusion protein 6B11mGM as anti-idiotype vaccines a gainst ovarian carcinoma.
出处
《解剖学报》
CAS
CSCD
北大核心
2000年第z1期101-105,共4页
Acta Anatomica Sinica
基金
教育部优秀年轻教师基金资助项目
关键词
卵巢癌
抗体
抗独特型
肿瘤疫苗
动物
实验
Ovarian carcinoma
Antibody/anti-idioype
Tumor vaccine
Animal
Laboratory
