摘要
目的探讨抗氧化剂硫辛酸(lipoicacid,LA)对氧化低密度脂蛋白(ox-LDL)引起的大鼠主动脉血管平滑肌细胞(VSMC)增殖的影响及其机制。方法将培养的大鼠主动脉平滑肌细胞分成5组:对照组、ox-LDL组及LA三个浓度干预组(20、80和100mg/L)。MTT比色法测定VSMC增殖程度,生化法测定丙二醛(MDA)及一氧化氮(NO)含量。结果与对照组相比,ox-LDL组大鼠主动脉VSMC增殖明显,MDA水平升高,NO水平降低,差异均有统计学意义(P<0.05～0.01)。与ox-LDL组比较,LA各浓度干预组(20、80和100mg/L)大鼠主动脉VSMC增殖受到抑制,MDA水平下降,NO水平升高,差异均有统计学意义(P均<0.05)。LA各浓度干预组间VSMC增殖程度、MDA和NO水平差异亦有统计学意义(P<0.05)。结论LA对ox-LDL引起的大鼠主动脉VSMC的增殖有抑制作用,而且可同时使细胞内MDA减少及NO含量增加,从而有可能延缓动脉粥样硬化的发生发展过程。
Objective To investigate the influence and the mechanisms of antioxidant lipoic acid on the proliferation of rats aorta vascular smooth muscle cells (VSMC) induced by ox-LDL. Methods The cultured rats aorta VSMC were divided into five groups,untreated control group,ox-LDL group and three groups with different dose of lipoic acid(20mg/L,80mg/L and 100mg/L). Methyl thiazolyl tetrazolium(MTT) assay and biochemistry detection were used to determine the effect of lipoic acid on anti-proliferation and the levels of malondialdehyde(MDA) and nitric oxide(NO) in the cultured rat aortic VSMC induced by ox-LDL. Results It was found that the proliferation of VSMC and the level of MDA in ox-LDL group were both higher than those in the control group(P<0.01,P<0.05),and the level of NO was lower than that in the control group. Compared with ox-LDL group,three groups with different dose of lipoic acid had lower level of proliferation of VSMC and MDA and higher lever of NO(P<0.05). Furthermore,among the three groups with different dose of lipoic acid there were significantly different in the level of proliferation of VSMC,the level of MDA and NO(P<0.05). Conclusion Lipoic acid can not only inhibit the proliferation of rat aortic VSMC induced by ox-LDL,but also lower the amount of MDA and enhance the amount of the NO in VSMC,that may be the mechanisms of delaying the development of atherosclerosis.
出处
《中国卒中杂志》
2008年第3期169-173,共5页
Chinese Journal of Stroke
关键词
硫辛酸
抗氧化剂
胆固醇
LDL
肌
血管
平滑
Lipoic acid
Antioxidants
Cholesterol,LDL
Muscle,vascular,smooth
