摘要
目的分析健康成人、儿童和患者3组人群中分离的大肠埃希菌对常用抗菌药物的耐药性和耐药机制。方法采用MicroScan-Walkway-40系统鉴定细菌,琼脂平皿倍比稀释法测定各种抗菌药物MIC(minimal inhibitory concentraton),对筛选出的215株MDR进行PCR扩增及序列分析检测这些菌株产AmpC和ESBLs的基因型。结果大肠埃希菌对亚胺培南、阿米卡星、哌拉西林/他唑巴坦显示较好的敏感性。健康成人和临床患者携带的MDR比例分别为58.3%和74.6%。PCR及序列分析表明本地区已出现CMY-2、DHA-1型AmpC酶和以CTX-M为主的ESBLs,并检测到1株新CMY型基因和1株ESACs菌株。结论健康人与患者肠道中病原大肠埃希菌对常用抗菌药物的耐药率相当高,在MDR中检测出多种AmpC和ESBLs基因。应严格控制抗菌药物使用并养成良好卫生习惯以减少耐药株出现。
Objective To investigate the difference of antibiotic susceptibility and multiresistant mechanism in Escherichia coli isolated from stool and cilnical samples collected from the healthy human and patients.Methods The bacteria were identified by MicroScan-Walkway-40 system.Agar dilution method was used to determine MIC(minimal inhibitory concentraton) of 14 antibiotics.AmpC and ESBLs genotypes were analyzed by PCR amplification and product sequencing.Results Imipenem/cilastain,amikacin and piperacillin/tazobactam had good activity against Escherichia coli.The multiresistance rates of the healthy human and patients were 58.3% and 74.6%,respectively.PCR amplification and sequencing showed that blaCMY-2,blaDHA-1 and CTX-M ESBLs were detected.A new CMY genotype and one ESACs were detected.Conclusions Higher resistance rate were detected in healthy human and patients.AmpC and ESBLs gene were produced by multiresistant Escherichia coli.More judicious use of antibiotic and better health habit may decrease the incidence of resistant Escherichia coli.
出处
《中华实验和临床感染病杂志（电子版）》
CAS
2008年第2期13-20,共8页
Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)
基金
安徽省卫生厅基金资助项目(05A037)
安徽省自然科学基金项目(30571654)
关键词
大肠埃希菌
耐药性
人群
Escherichia coli
Resistance
Human
