摘要
在生理pH下,采用光谱法、电化学、化学热力学和粘度法等方法,利用中性红作分子探针,研究了刚果红与鲱鱼精DNA之间的作用方式,获得了结合比、结合常数和热力学函数值。结果表明,刚果红与DNA之间以嵌插方式发生作用。刚果红与DNA之间的结合比为n(CR)∶n(DNA)=1∶1,结合常数为K 25℃=4.50×103L/mol。25℃时,ΔrHm=7.04×104J/mol,ΔrSm=305.88 J/(mol.K),ΔrGm=-2.08×104J/mol。刚果红与DNA之间的作用为熵驱动。
Under the condition of physiological pH,the interaction between Congo red and DNA was studied by spectroscopic,electrochemical,thermodynamic and viscometry methods,using Nature red as molecular probe.Binding stoichoimetry,binding constant and the corresponding thermodynamic parameters were obtained.The results indicated that the interaction of Congo red with DNA is intercalation binding.The binding stoichoimetry of n(CR)∶n(DNA) is 1∶1,and the binding constant of K25℃ is 4.50×103 L/mol.The thermodynamic para...
出处
《四川大学学报（工程科学版）》
EI
CAS
CSCD
北大核心
2009年第5期97-102,共6页
Journal of Sichuan University (Engineering Science Edition)
基金
国家自然科学基金资助项目(30572254)
