摘要
目的探讨慢性碘超足量和碘过量对Wistar大鼠甲状腺细胞死亡和细胞周期的影响。方法将Wistar大鼠按日摄碘量分为对照组(碘适量组,4μg/d)、1.5倍组(碘超足量组,6μg/d)、3倍组(3倍碘过量组,12μg/d)和6倍组(6倍碘过量组,24μg/d)。各组大鼠分别于实验0、1、2、4、8个月处死,采用砷铈催化分光光度法测定尿碘浓度,应用碘化丙锭染色流式细胞术测定细胞死亡率和细胞周期。结果3倍组和6倍组在4个月和8个月时,甲状腺细胞死亡率升高(17.4%vs9.8%,P<0.05),S期细胞比例升高(5%～6%vs3%,P<0.05),G0G1期细胞数显著下降(64%～67%vs80%,P<0.05)。尿碘浓度与细胞死亡率、S%显著正相关(r=0.673,r=0.505,P均<0.01),与G0G1%显著负相关(r=-0.639,P<0.01)。结论慢性碘过量增加甲状腺细胞的死亡率,并影响G0G1期和S期细胞比例。
Objective To investigate the chronic effect of more than adequate and excessive iodine on cell death and cell cycle in Wistar rat thyrocytes.Methods According to daily iodine intake,120 Wistar rats were divided into control group(adequate iodine,4 μg/day),group 1.5T(more than adequate iodine,6 μg/day),group 3T(excessive iodine,12 μg/day),and group 6T(excessive iodine,24 μg/day).The rats were killed 1,2,4,and 8 months after idione exposure.Urinary iodine concentration was measured by arsenic/cerium catalyzin...
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2009年第11期801-803,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目(30240013)
辽宁省博士科研启动基金资助项目(20071043)
