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白细胞介素2提高HBV基因疫苗诱导的体液免疫应答 被引量:8

Interleukin-2 promotes the specific humoral immune response induced by hepatitis B viral gene vacdne
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摘要 目的观察IL-2真核表达载体对HBV基因疫苗诱导BALB/c小鼠的特异性体液免疫应答的影响。方法肌肉注射基因疫苗及IL-2真核表达载体,ELISA法检测小鼠血清抗-HBs。结果免疫8周后,单纯注射pCR3.1-S及共注射IL-2真核表达载体的小鼠血清450nm A值分别为1.24±0.10及1.98±0.17,两组相比有明显差异(P<0.01)。结论IL-2的真核表达载体能够提高小鼠对基因疫苗的体液免疫应答。 Objective To observe the effect of interleukin-2 eukaryotic expression plasmid on specific humoral immune response to gene vaccines coding HBV surface antigen in Balb/c (H - 2d) mice. Methods The immunization was performed by intramuscular injection in this experiment. Anti-HBs in serum was detected by ELISA. Results 8wks after immunization, the value of 450nm of mouse sere codelivered with IL-2 vaccine (1.98 ± 0.17)was significantly higher than that of mice injected HBV - S gene vaccine alone (1.24 ±0.10). Conclusion The results showed that the HBV gene vaccine( pCR3.1 - S) had strong antigenecity which can be promoted by vector coding murine IL-2.
出处 《实用肝脏病杂志》 CAS 2003年第2期78-79,共2页 Journal of Practical Hepatology
基金 国家自然科学基金资助项目(39770665)
关键词 基因疫苗 乙型肝炎表面抗原 IL-2 小鼠 Gene vaccine Hepatitis B surface antigen Interieukin 2 Mice
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  • 1杜德伟,周永兴,冯志华,姚志强,李光玉.IL-12及HBV基因疫苗共同免疫小鼠的效果[J].世界华人消化杂志,2000,8(2):128-130. 被引量:20
  • 2[2]Sin JI, Kim JJ, Arnold RL, et al. IL-12 gene as a DNA vaccine adjuvant in a herpes mouse model: IL-12 enhances Th1 - type CD4 +T cell - mediated protective immunity against herpes simplex virus -2 challenge. J Immunol, 1999,162(5) :2912 ~ 2921
  • 3[3]Nunberg JH, Doyle MV, York SM, et al. Interleukin 2 acts as an ad -juvant to increase the potency of inactivated rabies virus vaccine.Proc Natl Acad Sci USA, 1989,86(11) :4240 ~ 4243
  • 4[4]Chow YH, Huang WL, Chi WK, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin - 2. J Virol, 1997,71( 1 ): 169 ~ 178
  • 5[5]Geissler M, Gesien A, Wands JR. Inhibitory effects of chronic ethanol consumption on cellular immune responses to hepatitis C virus core protein are reversed by genetic immunizations augmented with cytokine - expressing plasmids. J Immunol. 1997, 159(10):5107 ~ 5113

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