摘要
目的 观察噪声暴露对豚鼠耳蜗内毛细胞谷氨酸样免疫反应的影响 ,了解噪声暴露后耳蜗内毛细胞传入神经递质谷氨酸的释放情况 ,进而证实谷氨酸的过度释放是噪声暴露后耳蜗内毛细胞下传入神经树突损害的原因。 方法 将实验动物随机分为正常对照组与噪声暴露后 8h组。采用免疫细胞化学 SP染色法 ,结合计算机图像分析方法 ,比较两组动物耳蜗内毛细胞谷氨酸样免疫阳性反应的平均光密度 (AOD)值。 结果 豚鼠耳蜗内毛细胞谷氨酸样免疫阳性反应的 AOD值 ,在噪声暴露后 8h组明显小于正常对照组 (P<0 .0 1)。 结论 噪声暴露可引起耳蜗内毛细胞传入神经递质谷氨酸的过度释放 ,是引起耳蜗内毛细胞下传入神经树突损害的原因之一。
Objective To study the effects of noise exposure on glutamate like immunoreactivity(Glu IR) of inner hair cells(IHCs) in guinea pigs, and explore the injury mechanism of the afferent nerve endings underlying cochlear IHCs caused by noise with respect to the excitotoxicity of the excessive afferent neurotransmitter glutamate in cochlea. Methods 14 guinea pigs served as subjects. The experimental animals were randomly distributed into 2 groups: a normal control group, and a experimental group of 8 hours after noise exposure. The experimental group were immersed in 120 dB SPL one third octave 4 kHz narrow band noise for 4 hours. The 2μm semithin sections of Corti's organ in the first turn of both sides cochlear basilar membrane from 2 groups were examined by immunochemical streptavidin peroxidase conjugated(SP) method and analysed by computer image. Results Compared with the normal control group (t test), the average optical density(AOD) of Glu IR in IHCs of the experimental animals reduced significantly(p<0.001). Conclusion The over release of afferent neurotransmitter glutamate in cochlea can be caused by noise exposure, and the excitotoxicity of the excessive glutamate may be one of the injury mechanisms for afferent nerve endings underlying IHCs in cochlea after noise exposure.
出处
《空军总医院学报》
2001年第4期191-193,共5页
Journal of General Hospital of Air Force,PLA
关键词
谷氨酸
耳蜗
毛细胞
内
免疫化学
噪声
Glutamate
Cochlea
Hair cells, inner
Immunochemistry
Noise
