摘要
目的 研究建立一种快速、灵敏、简便的测定污染物急性毒性的生物传感器。方法 利用明亮发光杆菌的细胞发光作为毒性的判断指标,将细胞固定化技术、生物传感器技术与发光细菌毒性测试技术有机结合起来,构建了一种细菌发光传感器。结果 在pH=7.0,温度20℃,3.0％ NaCl底液条件下,固定化菌膜发光强度达2～4nw,稳定发光时间60～80min,毒性测试结果EC_(50)(EC_(50):菌膜发光强度抑制50％所需受试物的浓度)如下:Hg^(2+)0.14mg/L,Cu^(2+)20.3mg/L,Zn^(2+)120mg/L,苯酚48.9mg/L,乐果5.26mg/L,乙醛210mg/L,与哺乳动物毒性试验的LD_(50)(大鼠,经口)具有相关性:Log LD_(50)=1.226+0.91 Log EC_(50),r=0.95。结论 不同毒物之间对细菌发光反应的抑制速率有差异。
Object This study established a rapid, sensitive and simple detecting technology, which uses biosensor to detect acute toxicity of pollutant. Method Depends on the detection of cellular light of photobacterium phosphoreum. We integrated the technique of fixing cells, and the technique of biosensor with the technique of detecting toxicity of luminous bacillus, and designed a kind of Bacterial Luminescent Biosensor. Result The result indicates that: under the situation of pH 7.0, temperature 20 centigrade, and 3.0% NaCl solution, the light intensity of the settled bacterial film is 2- 4nw, and the time that the bacteria can keep stable light is 60-80 minutes. The result of toxicity test EC50 is that: the consistence of Kg2+ is0.14mg/L, Cu2+ is20.3mg/L, Zn2+ is 120mg/L, phenol is 48.9mg/L, cygon is5.26mg/L, and acetaldehyde is 210mg/L. The toxicity test result EC50 correlates with LD50 of the mammalian toxicity test (rat, from mouth) as follow: Log LD50=1.226 + 0.91Log EC50, r= 0.95. Conclusion The light velocity of different bacteria is different from each other.
出处
《中国卫生工程学》
CAS
2002年第2期65-68,共4页
Chinese Journal of Public Health Engineering
基金
黑龙江省科委攻关项目
