摘要
目的 对14例不同年龄取材人骨组织行成骨细胞原代培养,观察其生长及碱性磷酸酶(AKP)活性,为骨科疾病及组织工程学的研究寻找适宜的细胞来源。方法 取材于引产胎儿颅盖骨6例、青年及中年髂骨各4例,改良联合法培养,观察细胞形态及生长情况,Von kossa及AKP组化染色法鉴定细胞,AKP活性检测,比较不同年龄的取材及不同活性的胎儿细胞生长速度及AKP活性的差异。结果 改良联合培养法可在短时间内获得大量高纯度成骨细胞,细胞生长速度与取材年龄呈负相关,AKP含量由大到小顺序为青春期>胎儿>中年;细胞生长情况与胎儿产出后情况相关。结论 青春期来源的成骨细胞更适用于组织工程骨重建的需要,成骨细胞的成骨活性与人体身高增长趋势吻合。
Objective The culture of osteoblast-like cells of human origin has become an important experimental model in the study of bone bioloyg; primary culture of osteoblast like cells obtained from 14 human donors of various ages was carried out for the study of the cell, growth and alkaline phosphatase(AKP) activities. Methods Osteoblast like cells were isolated from 6 fetal calvarium(age 20-38 months) ,4 youth (age 10-20y)and 4 middle aged(age 35-50y) iliums. Cells was obtained by digestion and their figures and growth were observed and identitied by Von kossa and AKP biochemical staining. Detected The AKP activities was detected and the growing speed and AKP activities of different age donors and different abortive time fetuses. were compared. Results The modified osteoblast digestion and culture was able to release a large amount of pure osteoblats in a short period A negative correlation beteen cells growing speed and donors ages was detected The endocellular AKP content existed in a descending order of : in the youth > in the fetus > in the middle aged. Conclusions The osteoblasts of youngters were more compatible with tissue - engineering remodeling,and the cells biologic activities accorded with the increase of human stature…
出处
《中国骨肿瘤骨病》
2002年第3期165-168,共4页
Chinse Journal Of Bone Tumor And Bone Disease
