摘要
目的 从细胞生物学及分子生物学水平探讨中药保妇康抗癌的作用机制。方法 以不同浓度的含药培养液作用于感染HPV16的人宫颈癌细胞系—SiHa细胞 ,并设立无药对照 ,通过细胞计数、MTT及流式细胞分析技术检测该药对细胞生长及增殖的影响 ;以RT -PCR法半定量检测对HPV16E6E7基因表达的影响。结果 经含有保妇康的培养液作用后 ,细胞数量减少 ,倍增时间延长 ,细胞存活率降低 ;细胞周期各时相中 ,S期所占比例减少 ;HPV16E6E7mRNA的表达量减少。结论 以莪术油为主要成分的保妇康不仅可直接抑制宫颈癌细胞的生长 ,并可能通过抑制HPV16E6E7基因表达的分子机制 ,达到抑制肿瘤目的。
Objective To investigate the molecular mechanism of Baofukang suppository, made from Chinese traditional herbs, as a potential inhibitor for cervical carcinoma cell growth. Methods The human cervical carcinoma cell line-SiHa was cultured with different concentrations of the liquid form of Baofukang suppository.Cytometry, MTT assay and flow cytometry assay(FCM)were then used to monitor the growth and proliferation of the cell line.The expressions of HPV16/E6E7 genes were simultaneously evaluated by reverse transcription-polymerase chain reaction(RT-PCR). Results Under the influence of Baofukang suppository which has Curcuma as main component, the growth of cells slowed down, the doubling time became longer and the livability of the cells fell down with lesser cells in the S state.At molecular level, decreased expressions of HPV16/E6E7 mRNAs were observed. Conclusions Baofukang suppository can inhibit the cervical carcinoma cell directly and also restrain the tumor by depressing the expression of HPV16/E6E7 genes.
出处
《中国妇产科临床杂志》
2003年第5期336-338,381,共4页
Chinese Journal of Clinical Obstetrics and Gynecology
基金
首都发展基金 (ZD199911)资助
关键词
保妇康
宫颈癌
人乳头状瘤病毒
Baofukang suppository, cervical carcinoma, human papillomavirus
