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环氧化酶-2抑制剂与抗癌药对Caco-2细胞抑制的相互作用

Interaction of inhibiting effects of cyclooxygenase-2 inhibitors and anticancer agents on Caco-2 cell strain
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摘要 目的:探讨环氧化酶-2(COX-2)抑制剂与细胞毒抗癌药对人结肠腺癌细胞Caco-2抑制的相互作用。方法:用MTT法测定尼美舒利和塞来昔布与细胞周期非特异性药物CDDP和细胞周期特异性药物5-Fu并用时Caco-2细胞的抑制作用。结果:塞来昔布1mg/L~10mgL与CDDP 1mg/L合用或塞来昔布1mg/L~5mg/L与5-Fu 0.5mg/L合用,对Caco-2细胞的抑制有相加作用。更高浓度(15mg/L~20mg/L)的塞来昔布与CDDP 1mg/L合用或塞来昔布10mg/L~20mg/L与5-Fu 0.5mg/L合用,引起拮抗作用。尼美舒利1mg/L~20mg/L与CDDP 1mg/L合用,对Caco-2细胞的抑制呈拮抗作用,而与5-Fu 1mg/L合用,对Caco-2细胞的抑制呈相加作用。结论:低浓度的塞来昔布和CDDP合用,呈相加作用,但当塞来昔布的浓度增大时,就转为拮抗。尼美舒利和CDDP合用时,无论浓度高低,都呈拮抗作用。 Objective:To explore interaction of inhibiting effects of cyclooxygenase-2 (COX-2)inhibitors and anticancer drugs on human colon carcinomas cells stain,Caco-2.Methods:To observe the inhibiting action of COX-2 inhibitors and cytotoxic drugs on Caeo-2 cells by MTT assay. Results:Mter dosing 1 mg/L~10 mg/L celecoxib and CDDP (Cisplatin)1 mg/L,addition effects were observed in inhibiting action of Caco-2 strain, but antagonism was observed after dosing higher concentration of celeeoxib (15 mg/L~20 mg/L)and CCDP 1 mg/L or co-administering celecoxib 10 mg/L~20 mg/L and 5-FU 0.5 mg/L. Antagonism was observedfollowingdosingnimesulide 1 mg/L~20mg/L and CCDP to Caco-2 cell strain,whereas additivity showed after dosing nimesulide 1mg/L~20mg/L and 5-FU 1mg/L. Conchusion:Additive interactions were observed when dosing low concentration of celecoxib and CDDP to Caco-2 cell strain,and when the concentration of celecoxib was increased, the interactions turn into antagonism. Antagonism was observed when dosing nimesulide and CDDP,regardless of nimesulide concentration.
出处 《中国药物应用与监测》 CAS 2004年第4期55-57,共3页 Chinese Journal of Drug Application and Monitoring
关键词 尼美舒利 塞来昔布 CDDP 5-Fu 相互作用 Nimesulide Celecoxib CDDP 5-Fu Interaction
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  • 1Yun-Lin Wu~1 Bo Sun~1 Xue-Jun Zhang~2 Sheng-Nian Wang~2 Heng-Yi He~2 Min-Min Qiao~1 Jie Zhong~1 Jia-Yu Xu~1 1 Department of Gastroenterology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China2 Institude of Biochemistry and Cell Biology,Shanghai Institues for Biological Sciences,Chinese Academy of Sciences.Shanghai 200025,China.Growth inhibition and apoptosis induction Sulindac on Human gastric cancer cells[J].World Journal of Gastroenterology,2001,7(6):796-800. 被引量:64
  • 2吴军正,司徒镇强,刘斌,王为,陈建元.在抗癌药物敏感性试验中MTT法和MINI法的比较[J].实用口腔医学杂志,1994,10(1):55-56. 被引量:3
  • 3金正均.合并用药中的相加[J].中国药理学报,1980,1:70-73.
  • 4Kune GA, Kune S, Watson LF. Colorectal cancer risk, chronic illnesses, operations, and medications: case control results from the Melbourne Colorectal Cancer Study. Cancer Res, 1988; 48:4399~4404.
  • 5Harris RE, Namboodiri KK, Farrar WB. Nonsteroidal antiinflammatory drugs and breast cancer. Epidemiology, 1996,7:203~205.
  • 6Harris RE, Kasbari S, Farrar WB. Prospective study of nonsteroidal anti-inflammatory drugs and breast cancer. Oncol Rep, 1999; 6:71~73.
  • 7Phillips RK, Wallace MH, Lynch PM, et al. A randomized, double blind, placebo controlled study of celecoxib, a selective cyclooxygenase 2 inhibitor, on duodenal polyposis in familial adenomatous polyposis. Gut, 2002; 50:857~860.
  • 8Pentland AP, Schoggins JW, Scott GA, et al. Reduction of UVinduced skin tumors in hairless mice by selective COX-2 inhibition.Carcinogenesis, 1999; 20:1939~1944.
  • 9Grubbs C J, Lubet RA, Koki AT, et al. Celecoxib inhibits N-butyl-N(4-hydroxybutyl)- nitrosamine-induced urinary bladder cancers in male B6D2F1 mice and female Fischer-344 rats. Cancer Res, 2000;60:5599~5602.
  • 10Hsueh CT, Chiu CF, Kelsen DP, et al. Selective inhibition of cyclooxygsnase-2 enhance mitomycin-C-induced apoptosis. Cancer Chemother Pharmcol, 2000; 45:389~396.

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