摘要
目的 研究肝癌组织、癌旁组织端粒酶活性的表达及DNA含量的变化。方法 取肝癌手术标本 30例 ,每例均取癌组织、癌旁肝组织及外侧切缘肝组织。采用PCR -ELISA法检测其端粒酶的活性 ;流式细胞仪技术检测细胞DNA含量 ,DNA倍体及细胞分期。结果 肝癌组织端粒酶阳性率为 76 7% (2 3 30 ) ,癌旁组织端粒酶阳性率为 16 7% (5 30 ) ,外侧切缘肝组织无阳性 (0 30 ) ,肝癌组织与癌旁组织及外侧切缘肝组织端粒酶的表达有显著差异 (P <0 0 1)。癌旁组织与外侧切缘肝组织端粒酶的表达也有显著差异 (P <0 0 5 )。肝癌组织与外侧切缘肝组织DNA异倍体表达率有显著差异 (P <0 0 5 )。端粒酶阳性及阴性组间DNA异倍体率、DNA指数 (DI)、S期细胞比率 (SPF) ,细胞增殖指数 (PI)未见明显变化。结论 端粒酶与肿瘤的恶性行为存在一定的密切关系 ,其作为肝癌诊断的分子标志具有一定的价值。同时检测DNA倍体类型可进一步提高其临床参考价值。
Objective To investigate the telomerase activity and change of DNA in liver cancer.Methods telomerase activity was analyzed in 30 1iver cancers using PCR-ELISA;DNA gel electrophoresis and flow cytometry(FCM)were detected.Results Of these patients,the positive rate of telomerase activity was 76.7%(23/30),in cancerous tissues,16.7%(5/30)in juxtacancerous tissue and no positive expression of telomerase(0/30)in liver tissues.The telomerase activity between cancerous tissues and juxtacancerous tissues and liver tissues was significantly difference(P<0.01).The telomerase activity between juxtacancerous tissue and liver tissues was significantly difference(P<0.05).DNA between cancerous tissues and liver tissues was significantly difference(P<0.05)DNA,DI,SPF in tissues between positive and negative of telomerase activity have not change.Conclusion The telomerase activity with detected DNA would be a diagnosis marker for liver cancer
出处
《癌症进展》
2004年第5期378-380,共3页
Oncology Progress
基金
福建省科技项目资助 99-Z -14
关键词
肝癌
端粒酶
DNA
liver cancer telomerase DNA
