摘要
目的研究良恶性胸腔积液中间期细胞遗传学方面的差异,以及FISH技术对此种差异的鉴别能力。方法采用7号、8号人染色体着丝粒特异性探针对21例临床诊断明确患者的胸腔积液(11例肿瘤患者,10例非肿瘤患者)进行FISH分析,计数超二倍体细胞的比例,以正常人外周血淋巴细胞作为对照来判定染色体数目的异常,并将结果与临床诊断进行比较。结果在正常对照的淋巴细胞中,7、8号人染色体着丝粒探针各出现2个杂交信号,肿瘤患者胸腔积液细胞中7、8号人染色体数目的变化主要表现为拷贝数增多。在11例恶性胸腔积液中,出现7、8号人染色体数目增多的例数分别为8(72.7%)和9(81.8%),10例良性胸腔积液中7、8号人染色体为正常二倍体的各有9例(90.0%)。结论7、8号人染色体超二倍体改变是肿瘤患者胸腔积液细胞的主要特征,采用染色体着丝粒特异性探针的荧光原位杂交技术能够检测到胸腔积液标本中的超二倍体肿瘤细胞,并且具有较好的性能。
Objective To study the interphase cytogenetic difference between benign and malignant pleural effusion as well as the differential capability of fluorescence in situ hybridization (FISH) of such difference.Methods FISH analysis was performed with centromere DNA probes of chromosome 7and 8 upon 21 clinically collected pleural effusion samples,each with clear diagnosis,and the percentage of hyperdiploid cells was calculated,taking normal lymphocytes in peripheral blood as the control.In addition,the result was also compared with relevant clinical diagnosis.Results In the normal lymphocytes control,2 hybridization signals were observed for each centromere DNA probe,while hyperdiploid presented in the pleural effusion from tumor patients.Hyperdiploid of chromosomes 7 and 8 were respectively found in 8(72.7%) and 9(81.8%) of 11 malignant pleural effusion samples.While for 10 benign pleural effusion samples,9(90.0%) normal diploid samples were confirmed respectively by each centromere DNA probe.Conclusion Hyperdiploid of chromosomes 7 and 8 are the main characteristic of pleural effusion cells from tumor patients.FISH analysis with centromere DNA probes of chromosomes 7 and 8 could detect the hyperdiploid tumor cells in pleural effusion samples with excellent performance.
出处
《癌症进展》
2005年第5期500-505,共6页
Oncology Progress
关键词
荧光原位杂交
胸腔积液
超二倍体
fluorescence in situ hybridization pleural effusion hyperdiploid
