摘要
目的了解鲍曼不动杆菌(ABA)耐药性和氨基糖苷类修饰酶基因(AME)存在状况。方法用K-B法测定ABA对14种抗菌药物的敏感性,用PCR法检测6种AME和Ⅰ类整合酶基因(intⅠ1)。结果85株ABA的耐药率除头孢哌酮/舒巴坦(8.2%)和阿米卡星(18.8%)外,其余12种抗菌素均在50%以上(48.2%～81.2%),其中妥布霉素、奈替卡星和庆大霉素的耐药率分别达55.3%、48.2%和70.6%。耐药基因检测ant(3″)-Ⅰ阳性61株(71.8%)、aac(6')-Ⅰb阳性53株(62.3%)、aac(3)-Ⅰ阳性39株(45.9%),intⅠ1阳性66株(77.6%),其余基因均阴性。锚定PCR扩增结果表明ant(3″)-Ⅰ、aac(3)-Ⅰ型AME在Ⅰ类整合子上。结论绍兴地区临床分离的ABA多重耐药情况十分严重,对氨基糖苷类抗生素耐药主要由ant(3″)-Ⅰ、aac(6')-Ⅰb、aac(3)-Ⅰ基因介导,Ⅰ类整合子在耐药性传播中起着非常重要的作用。
Objective To investigate the correlation of the drug resistance and genes of aminoglycoside modifying enzymes (AME) in A.baumannii. Methods K-B tests were performed to detect the susceptibilities of 14 kinds of antimicrobial agent for 85 strains of A.baumannii. The AME coding gene was analyzed by PCR. Results The resistant rates of the antimicrobial agents showed the susceptibilities between 8.2% and 81.2%. The detective rates of AME coding gene of ant(3″)-Ⅰ, aac(6')-Ⅰb, aac(3)-Ⅰ were 71.8%, 62.3% and 45.9%, respectively. intⅠ1 was 77.6%. Genes of aac(3)-Ⅱ, aac(6′)-Ⅱ and ant(2″)-Ⅰwere not found. Conclusions The multi-resistance of A.baumannii showed very high in Shaoxing. AME of ant(3″)-Ⅰ, aac(6')-Ⅰb and aac(3)-Ⅰ are the important mechanism for drug resistance, and which has the important role in transmitted of A.baumannii.
出处
《实验与检验医学》
CAS
2007年第6期533-535,共3页
Experimental and Laboratory Medicine
关键词
鲍氏不动杆菌
耐药性
氨基糖苷类修饰酶
基因
Acinetobacter baumannii
Drug-resistance
Aminoglycoside modifying enzymes
Genes
