摘要
采用单磺酰氯柱前衍生高效液相色谱法测定乳制品中甘氨酸的含量。应用单磺酰氯作为柱前衍生试剂,55℃温度下,反应15min。衍生产物用C18柱(3.9mm×150mm,5μm)分离,0.1mol/L醋酸钠(pH7.2):乙腈75:25为流动相进行洗脱,流速1.0mL/min,在330nm的波长下,能准确检测出乳制品中游离甘氨酸。方法回收率在98.2%～102%之间,在0～205mg/L范围内,线性方程相关系数为0.99994,相对标准偏差RSD为0.87%。该方法衍生反应简单,时间短。衍生产物在330nm具有较强的紫外吸收,且产物单一稳定,冰箱中存放可稳定一星期以上,分析速度快,7min内可与其他峰完全分离。本方法具有操作简单?结果重复性好?准确等优点。
The glycin in dairy products was determined by dansyl chloride as the pre-column derivation reagent at 55℃ for 15min.The derivation can be detected easily and accurately by HPLC.The pre-column derivation was separated by C18 column(3.9 mm × 150mm,5μm),and eluted by mobile phase of 0.1mol/L sodium acetate(pH7.2)and acetonitrile with proportion of 75:25,detected at 330nm wavelength.In 7minutes,the derivation was effectively separated.The recovery was between 98.2%～102%,the correlation was 0.99994 with the range between 0～205mg/L.The derivation was a single product and had strong UV absorption at 330nm.It was stable for more than one week in the refrigerator.The method had the advantage of simple operation,good repetition and accuracy.
出处
《中国食品添加剂》
CAS
北大核心
2009年第4期169-172,共4页
China Food Additives
关键词
甘氨酸
单磺酰氯
柱前衍生色谱法
乳制品
glycin
Dansyl chloride
pre-column derivation
HPLC
dairy products
