摘要
实验采用DA201-C型大孔吸附树脂对Alcalase蛋白酶水解鹿茸血3h的水解液进行吸附,采用25%、50%、75%、100%乙醇分级洗脱,收集各组分进行氨基酸组成分析,发现各洗脱组分具有不同疏水性值,同时测定各组分的血管紧张素转化酶(ACE)抑制活性和二苯代苦味肼基自由基(DPPH.)清除活性:75%乙醇洗脱组分的ACE抑制活性最高,为54.71%,且ACE抑制活性与组分疏水性值显著相关;100%乙醇洗脱组分的DPPH?清除率最高。
Red deer antler blood hydrolysates were prepared by enzymatic hydrolysis with Alcalase for 3 h.Macroporous adsorption resin were used for the fractionation of hydrophobic peptides from the hydrolysates by gradient ethanol elution.Amino acid analysis results suggested that different ethanol elution fractions have different hydrophobicities.75% ethanol fraction showed the highest inhibitory activity of angiotensin I-converting enzyme (ACE),and the ACE inhibitory activity has a significant correlation with hyd...
出处
《天然产物研究与开发》
CAS
CSCD
2010年第2期302-306,共5页
Natural Product Research and Development
基金
国家自然科学基金项目(30571347)
863项目(2007AA10Z325)
