摘要
10-脱乙酰巴卡亭Ⅲ是合成紫杉醇和多烯紫杉醇的前体。以巴卡亭Ⅲ为底物,结合TLC、HPLC、HPLC-MS分析方法,通过设计专门的筛选方法筛选产酶菌株,得到一株巴卡亭ⅢC-10位脱乙酰基酶产生菌株Z1-56。以形态特征、生理生化特征、16S rDNA序列分析作为菌株的鉴定手段,Z1-56被鉴定为成团泛菌(Pantoea agglomerans),首次发现成团泛菌产生巴卡亭ⅢC-10-脱乙酰酶。
C-10 deacetyl baccatin Ⅲ(10-DAB)is the key precursor for synthesis of taxol and taxotere.A special medium was designed for screening C-10 deacetylase producing strains.Analyzed by TLC,HPLC,and HPLC-MS,A Z1-34 strain which can hydrolyze C-10 acetyl of baccatin III was obtained when bacctin III was added in fermentation broth.Identification by morphological characteristics,physiological and biochemical features and 1616S rDNA sequence analysis,strain Z1-56 was identified as Pantoea agglomeran.It is first foun...
出处
《天然产物研究与开发》
CAS
CSCD
2010年第1期72-76,共5页
Natural Product Research and Development
