摘要
利用ISSR技术对3个甜玉米组合及其父、母本自交系共9个材料进行鉴定,从30条引物中筛选出4条扩增条带清晰、多态性较高的引物,共扩增出31条DNA带,其中24条DNA带具有多态性,多态性比率为77.4%。对9个玉米材料的亲缘关系进行聚类分析,9个甜玉米材料分为两个类群。
To study purity identification of sweet corn seeds,ISSR markers were used to screen in 9 materials of sweet corn.Four primers were screened out from 30 ISSR primers totally to amplify the sweet corn genome DNAs of 9 materials effectively and 31 sites were amplified out totally,including 24 polymorphism sites with polymorphism rate of 77.4%.All of the 9 materials could be differentiated from each other based on the fingerprints established by these specific bands.The systematical cluster analysis conducted w...
出处
《玉米科学》
CAS
CSCD
北大核心
2010年第1期46-48,共3页
Journal of Maize Sciences
基金
广东省省级产业技术研究与开发项目[粤财工(2008)151号]
惠州市科技计划项目(2009B010001025)
关键词
甜玉米I
SSR
纯度鉴定
Sweet corn
ISSR
Purity identification
