期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

小麦农家品种白葫芦抗白粉病基因的SSR分析 被引量：4

SSR Analysis of Powdery Mildew Resistance Gene in Chinese Wheat Landrace Baihulu
下载PDF
导出
摘要 为明确小麦农家品种白葫芦抗白粉病基因的遗传特点和基因位点,采用常规分析方法结合SSR技术进行抗性基因的遗传分析和分子标记研究。结果表明,白葫芦与高感白粉病小麦品种陕225及辉县红的F1代幼苗均高抗白粉病,F2代抗感植株比例分别为112∶38和118∶42,经χ2检验,符合3∶1的遗传分离比例,说明白葫芦的苗期抗白粉能力由显性单基因控制。利用208对SSR引物在父母本及抗感池间进行多态性筛选,引物Xg-wm337与该抗白粉病基因紧密连锁,遗传距离为4.6 cM。利用这对引物对白葫芦/陕优225组合F2进行验证分析,结果与抗性调查结果基本相符。经中国春缺体-四体系分析,将该抗白粉病基因定位于1D染色体上,暂命名为mlbhl。 To definite the genetic effects and gene loci of powdery mildew resistance gene in Chinese wheat landrace Baihulu,conventional methods and SSR analysis was used to analyze the heredity and molecular markers of this resistant gene.The results showed that F1 of Baihulu×Huixianhong and Baihulu×Shaanyou 225 were highly resistant to powdery mildew,the ratios of resistance plants to susceptible plants in F2 generation were 118∶42 and 112∶38,respec-tively.The segregation ratio fitted the expected 3∶1,so it suggest...
作者 赵宁娟 薛飞 王长有 韩静然 吉万全 郑蕾
机构地区 西北农林科技大学农学院/国家小麦改良中心杨凌分中心 咸阳市农科所
出处 《麦类作物学报》 CAS CSCD 北大核心 2010年第3期411-414,共4页 Journal of Triticeae Crops
基金 国家"十一五"科技支撑计划项目(2006BAD13B02) 陕西省"13115"科技创新工程项目(2008ZDKG-07) 西北农林科技大学人才基金资助项目
关键词 小麦 农家种白葫芦 白粉病 抗性基因 SSR分析 Wheat Landraces Baihulu Powdery mildew Resistance gene SSR analysis
分类号 S512.1 [农业科学—作物学]
  • 相关文献

参考文献14

二级参考文献25

  • 1司权民，中国农业科学，1987年，20卷，5期，64页
  • 2Lander E S,Green P,Abrahamson J,Barlow A,Daly M J,Lincoln S E, Newburg L. MAPMAKER:an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations [J]. Genomics, 1987,1,174 ～ 181.
  • 3Wang S,Liu N,Peng K,Zhang Q. The distribution and copy number of copia-like retrotransposons in rice (Oryza sativa L. ) and their implications in the organization and evolution of the rice genome [J]. Proc Natl Acad Sci USA, 1999,96: 6824～6828.
  • 4Zeller F J, Hsam S L K. Progress in breeding for resistance to powdery mildew in common wheat ( Triticum aestivum L. ). In: McIntcsh R A, Hart G E, Devos K M, Gale M D, Rogers W J eds. Proc 92^th International Wheat Genetics Symp. Saskatoon,Canada: University Extension Press,University of Saskatchewan, 1998, pp 178-180.
  • 5R oder M S, Korzun V, Wendekhake K, Plaschke J, Tixier N H, Leroy P, Ganal M W. A microsatellite map of wheat. Genetics, 1998, 149: 2007-2023.
  • 6Konm V, Roder M S, Ganal M W, Worland A J, Law C N. Genetic analysis of the dwarfing gene ( Rht8 ) in wheat: Part Ⅰ . Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat ( Triticum aestivum L. ) . Theor Appl Genet, 1998, 96:1104-1109.
  • 7Chague V, Fahima T, Dahan A, Sun G L, Korol A B, Ronin Y I, Grama A, Roder M S, Nevo E. Isolation of microsatellite and RAPD markers flanking the Yr15 gene of wheat using NITs and bulked segregant analysis. Genome, 1999, 42:1050-1056.
  • 8Ma J X, Zhou R H, Dong Y S, Wang L F, Wang X M, Jia J Z. Molecular mapping and detection of the yellow rust resistance gene Yr26 in wheat transferred from Triticum turgodum L. using microsatellite markers. Euphytica, 2001, 120:219-226.
  • 9Wang L F, Ma J X, Zhou R H, Wang X M, Jia J Z. Molecular tagging of the yellow rust resistance gene Yr10 in common wheat, P Ⅰ 178383 ( Triticum aestivum L. ). Plant Breed, 2002, 124:71-73.
  • 10Bougot Y, Lemoine J, Pavoine M T, Barloy D, Doussinault G. Identification of a micresatenite marker associated with Pm3 resistance alleles to powdery mildew in wheat. Plant Breed, 2002, 121:325-329.

共引文献346

同被引文献60

引证文献4

二级引证文献15

麦类作物学报
2010年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部