摘要
目的观察东菱迪夫对脑缺血损伤后大鼠神经干细胞分化、迁移的影响,并探讨东菱迪夫治疗急性脑缺血损伤的可能机制。方法采用大脑中动脉阻塞法(MCAO)建立局灶性脑缺血再灌注模型;运用神经功能缺陷评分和TTC染色评定神经功能与脑梗死体积;免疫组化检测缺血侧脑室下区(SVZ)5-溴脱氧尿嘧啶核苷(BrdU)以及胶质纤维酸性蛋白GFAP(作为检测脑缺血后神经干细胞的分化、迁移的指标),从而评价东菱迪夫对缺血脑损伤后神经干细胞分化迁移的作用。结果东菱迪夫组(DLDF)再灌后第7天脑梗死灶体积明显小于模型组(P<0.01),DLDF组大鼠在第7、8、9、16天的神经功能缺陷评分明显优于模型组(P<0.05);和模型组相比,缺血损伤后第7天,东菱迪夫组大鼠SVZ的GFAP阳性细胞数明显增多(P<0.05);缺血损伤后第14天,东菱迪夫组大鼠SVZ的BrdU阳性细胞数明显增多(P<0.05),并且观察到从SVZ至其背外侧带的BrdU阳性细胞的迁移带。结论东菱迪夫能减少脑缺血大鼠的脑梗死灶体积和改善神经功能评分;缺血脑损伤后,脑内神经干细胞发生分化、迁移;东菱迪夫可促进SVZ神经干细胞的分化、迁移。
Objective To investigate the effect of Dong Ling Di Fu (DLDF) on neural stem cell differentiation and migration following acute cerebral ischemia in adult rats, and to explore the potential mechanisms. MethodsThe animal model of transient cerebral ischemia was induced by middle cerebral artery occlusion (MCAO).Neurological deficit was evaluated according to a five-point scale, cerebral affected size was measured by TTC stain. Expression of BrdU and GFAP (markers of neural stem cell differentiation and migra...
出处
《神经病学与神经康复学杂志》
2009年第3期208-211,219,共5页
Journal of Neurology and Neurorehabilitation
关键词
东菱迪夫
脑缺血损伤
神经干细胞
分化
迁移
Dong Ling Di Fu
cerebral ischemic injury
neural stem cell
differentiation
migration
